New Biomaging Tools
The brain is the most complex system known to us, and discovering the principles underlying its structure, dynamics and function poses one of the most important challenges to modern science. Despite great progress, we remain far from understanding how brain circuits create purposeful behavior by allowing animals and humans to perceive and predict the external world, store and retrieve memories, and calculate and execute decisions. The obstacle is that the computations of the brain lie in the collective dynamics of astronomically large numbers of components -- the integrated dynamics of billions of neurons and trillions of synapses. Understanding the brain will require new interdisciplinary approaches to map and analyze the complex structure, function and dynamics of brain circuits at both high spatial resolution and over large volumes, from the scale of individual neuronal synapses (~10 nm) to whole neurons (~10 microns) to complete circuits (~1 cm).
In our labs at the Harvard Center for Brain Science, we are developing and applying powerful new imaging tools for brain science -- including functionalized electron microscopy using multi-color cathodoluminescence to determine both the structure and function of brain circuits with individual synapse resolution; super-resolution optical imaging (STED) with the capability of nanoscale magnetic field sensing using NV-diamond (mag-STED) to provide real-time monitoring of the structure, dynamics and development of living neurons; and a wide-field-of-view diamond magnetic field imager to map individual neuron activity non-invasively in large (>1 mm) neuronal networks. The long-term goal is to understand the principles and computational significance of the topology, connectivity and development of brain circuits. Our efforts are closely collaborative with the group of Prof. Jeff Lichtman.
- Optical magnetic detection of single-neuron action potentials using quantum defects in diamond. J. F. Barry, M. J. Turner, J. M. Schloss, D. R. Glenn, Y. Song, M. D. Lukin, H. Park, and R. L. Walsworth. PNAS vol.113 no.49 (2016).
- Superresolution optical magnetic imaging and spectroscopy using individual electronic spins in diamond. J. Jaskula, E. Bauch, S. Arroyo-Camejo, M. D. Lukin, S. W. Hell, A. S. Trifonov, R. L. Walsworth. arXiv:1610.02097 (2016).
- Mapping the Microscale Origins of MRI Contrast with Subcellular NV Diamond Magnetometry. H. C. Davis, P. Ramesh, A. Bhatnagar, A. Lee-Gosselin1, J. F. Barry, D. R. Glenn, R. L. Walsworth, M. G. Shapiro. arXiv:1610.01924 (2016).
- Optical magnetic detection of single-neuron action potentials using quantum defects in diamond. J. F. Barry, M. J. Turner, J. M. Schloss, D. R. Glenn, Y. Song, M. D. Lukin, H. Park, R. L. Walsworth. arXiv:1602.01056 (2016).
- Efficiency of Cathodoluminescence Emission by Nitrogen-Vacancy Color Centers in Nanodiamond. Huiliang Zhang, David R. Glenn, Richard Schalek, Jeff W. Lichtman, Ronald L. Walsworth. arXiv:1602.00410 (2016).
- Single-cell magnetic imaging using a quantum diamond microscope. D. R. Glenn, K. Lee, H. Park, R. Weissleder, A. Yacoby, M. D. Lukin, H. Lee, R. L. Walsworth, C. B. Connolly, Nature Methods doi:10.1038/nmeth.3449 (2015).
- Supplementary material for the above article.
- Atomic-Scale Nuclear Spin Imaging Using Quantum-Assisted Sensors in Diamond. A. Ajoy, U. Bissbort, M. D. Lukin, R. L. Walsworth, P. Cappellaro. Physical Review X 5, 011001 (2015). arxiv:1407.3134.
- A Genetic Strategy for Probing the Functional Diversity of Magnetosome Formation. L. Rahn-Lee, M. E. Byrne, M. Zhang, D. Le Sage, D. R. Glenn, T. Milbourne, R. L. Walsworth, H. Vali, A. Komeili. PLOS Genetics 11 (1), e1004811 (2015).
- Silicon-Vacancy Color Centers in Nanodiamonds: Cathodoluminescence Imaging Markers in the Near Infrared. H. Zhang, I. Aharonovich, D.R. Glenn, R. Schalek, A.P. Magyar, J.W. Lichtman, E.L. Hu, and R.L. Walsworth. Small (2014). doi: 10.1002/smll.201303582.
- Limits to resolution of CW STED microscopy. A.S. Trifonov, J.C. Jaskula, C. Teulon, D.R. Glenn, N. Bar-Gill, and R.L. Walsworth. Advances In Atomic, Molecular, and Optical Physics 62, 279-302 (2013).
- Optical magnetic imaging of living cells. D. Le Sage, K. Arai, D. R. Glenn, S. J. DeVience, L. M. Pham, L. Rahn-Lee, M. D. Lukin, A. Yacoby, A. Komeili, and R. L. Walsworth. Nature 496, 486-489 (2013).
- Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours. D. R. Glenn, H. Zhang, N. Kasthuri, R. Schalek, P. K. Lo, A. S. Trifonov, H. Park, J. W. Lichtman, and R. L. Walsworth. Scientific Reports 2, 865 (2012). arxiv:1208:1249.
- Supplementary material for the above article.
- Magnetic field imaging with nitrogen-vacancy ensembles. L.M. Pham, D. Le Sage, P.L. Stanwix, T.K. Yeung, D. Glenn, A. Trifonov, P. Cappellaro, P.R. Hemmer, M.D. Lukin, H. Park, A. Yacoby and R.L. Walsworth. New Journal of Physics 13 045021 (2011).
- Far-field optical imaging and manipulation of individual spins with nanoscale resolution. P.C. Maurer, J.R. Maze, P.L. Stanwix, L. Jiang, A.V. Gorshkov, A.A. Zibrov, B. Harke, J.S. Hodges, A.S. Zibrov, D. Twitchen, S.W. Hell, R.L. Walsworth and M.D. Lukin. Nature Physics,6 912 (2010).